Jay Z hosted the charity show at the Barclays Centre in Brooklyn to celebrate the online music streaming service reaching one million subscribers and the event was streamed on Tidal.
The 'Drunk In Love' singer replaced Justin Timberlake's vocals on his and Jay Z's hit 'Holy Grail' and told the crowd it's the couple's ''favourite'' track to perform together.
If that wasn't enough, Queen B then joined 'Anaconda' rapper Nicki for two songs they've collaborated on, a highly energetic and raunchy performance of 'Feeling Myself' and 'Flawless', in which they wore sexy leotards flaunting their famous booty's.
Damian Marley also played on the night and was revealed to be the latest celebrity backer of Tidal.
Prince was also due to play at the concert, however, he pulled out without giving a reason for his absence.
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