The evening will be hosted by Sir Tom Jones who said he is pleased to have the artists on the show as they will each bring something individual to the evening of live music and comedy.
In a statement, Tom said: ''I am delighted that 5 Seconds of Summer, Dame Evelyn, Laura, James and The Shires are going to be joining us on October 8 for what is set to be a night of great music and fantastic comedy. All of them will bring something very different to the show, so I know people attending on the night will be in for a very special treat.''
While the 'She Looks So Perfect' rockers are ''really excited'' to be coming to London specially from Australia.
They commented: ''We are really excited to be coming to London to perform at 'One Big Night for BBC Children in Need,' it is going to be a great show, and all for a great cause. We look forward to seeing you all there!''
And 'Broken Strings' hitmaker James is ''thrilled'' to be supporting the show in aid of disadvantaged children.
He said of joining the line-up: ''BBC Children in Need is a fantastic cause and I am thrilled to be taking part in what is set to be a great evening amongst some amazing acts. Sir Tom and Rob are pulling together something really special, so I look forward to seeing you all there.''
The concert will be aired on BBC One on November 13.
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