The inaugural Unite4:Humanity event was created by bosses at charity Unite4:good and Variety magazine and was hosted by former Saturday Night Live comedian Andy Samberg.
De Niro received the Creative Commitment to Film award, Scorsese was given the Variety Creative Conscience gong and Samberg was handed the Keep Walking Award for his accomplishments.
Pop stars were also recognised for their charity work - Selena Gomez was named Young Humanitarian and Demi Lovato a Young Luminary, while Alicia Keys picked up a Music Visionary award. Both Keys and Lovato performed at the show.
Gomez took to Twitter.com after receiving the trophy, to write, "By far the coolest award I've received - Young Humanitarian award! Beyond proud to represent (charity) Unicef since I was 17."
Meanwhile, Lovato posted a picture of herself posing with former U.S. President Bill Clinton, who was the recipient of the Unity Recognition Award.
She tweeted: "Too excited to filter this... No big deal - selfies with the prez (president).... Bill Clinton - so nice to meet you!!!!"
Other attendees included Eva Longoria, Katherine Heigl, Dionne Warwick, Forest Whitaker and Philomena Lee, the woman whose story inspired acclaimed movie Philomena. She picked up an Everyday Hero award.