The guitarist, who has been a member of Bon Jovi since 1983, did not specify his reason for pulling out of the upcoming North American leg of the band's Because We Can trek.
A statement on the Bon Jovi website reads, "Due to personal issues, Richie Sambora will not be performing on this upcoming leg. All shows will go on as scheduled."
Sambora was missing when the Livin' on a Prayer hitmakers played the Scotiabank Saddledome arena in Calgary, Canada, on Tuesday (02Apr13) night.
Frontman Jon Bon Jovi told the crowd, "I had two choices - pack up and go home or give you everything I got. Richie Sambora won't be performing for a while. If there's ever a night I need you, it's tonight, Calgary!"
The singer is said to have promised to donate to a local charity to make amends for Sambora's absence.
Sambora has endured a turbulent few years which have included a stint in rehab, a drink-driving conviction, and a divorce from actress Heather Locklear.