The duo's Uptown Funk is the first single since the Black Eyed Peas' I Gotta Feeling in 2009 to spend at least 13 weeks at number one - and Mars and Ronson are just three weeks shy of matching Mariah Carey & Boyz II Men's 20-year record for the longest run on top of the Billboard Hot 100 countdown with One Sweet Day.
Maroon 5's Sugar, Ed Sheeran's Thinking Out Loud and Ellie Goulding's Love Me Like You Do stay put two, three and four, respectively, while The Weeknd enters the top five with Earned It (Fifty Shades of Grey).
Meanwhile, Kendrick Lamar is still all a flutter on top of the U.S. album chart after scoring a second week at number one with To Pimp a Butterfly.
Lamar holds on to his Billboard 200 lead to become only the second artist to land multiple weeks on top this year (15) - Taylor Swift is the other.
To Pimp a Butterfly keeps soundtracks from Empire and Fifty Shades of Grey off the top, while Sam Smith's In the Lonely Hour climbs back into the top five at four and Swift's 1989 stays put at number five.
Action Bronson's Mr. Wonderful and compilation Kidz Bop 28 are the only debuts in the new top 10, at seven and 10 respectively.