The actress, who walked free from court-ordered rehab last week (30Jul13), taped her appearance on comedian Chelsea Handler's show on Thursday (01Aug13) and poked fun at Kristen Stewart's dour personality in early teasers.
And now it appears she also takes shots at One Direction star Styles following a recent expose on the singer in Britain's GQ magazine.
During the accompanying interview, Styles laughed off reports suggesting he is bisexual and revealed that although he's a ladies man, he hasn't slept around.
And now he has to face the recharged Lohan's Mean Girls tongue.
In a new trailer for the show, which will air on Monday night (05Aug13), she says, "He was quizzed by the interviewer about rumours he's bisexual with a Dj named Nick. I've been there."
And Lohan, who famously dated gay Dj Sam Ronson, could not believe Styles has only slept with two women, as per his magazine confession.
She jokes, "He's never hung out with The Wanted and he's obviously not counting oral (sex)."
Lohan struck up a friendship with The Wanted's Max George last year (12) and was spotted partying with the wild boy band.