The 'Halo' singer's husband made sure she could use the rest room facilities at Hollywood vegan restaurant Crossroads in peace last week and stopped anyone from entering until his wife had finished.
It seems the 44-year-old rapper's chivalrous gesture didn't go down well with other diners who had to queue until Beyoncé was done.
An insider told Daily Star Newspaper: ''Halfway through their meal Jay got up and asked a waitress to check if anyone was currently in the bathroom.
''Once they had established the loos were empty, Beyoncé scurried inside while he stood guard over the door.
''When a couple of customers tried to go in, he politely asked them if they'd mind waiting until his wife was done. Some customers were a bit miffed, but Beyoncé was also embarrassed when she came out.''
The drastic actions may have seem irrational for some, but Beyoncé has to have privacy when she uses public toilets otherwise she gets constantly approached by fans at awkward moments.
The source added: ''She gets scared of getting accosted by someone who recognises her in public bathrooms. If she has a female friend with her, she always gets them to accompany her. But this time it was just her and her husband.''
Afterwards, Beyoncé and Jay Z reportedly signed autographs and posed for photos for fans who waited patiently.
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