The 'Not Afraid' rapper released a verse in new track 'A Kiss', in which he called the 'Born This Way' singer a "male lady", after rumours she was a hermaphrodite, but the rapper claims he still has respect for her.
He said: "I think Lady Gaga, as an artist you know, she's undeniable.
"That was one of them things where sometimes I get a joke in my head and I kinda want to share it with the world, you know what I'm saying.
"So it was just one of them things man, and it's you know it's all in fun, its hip-hop."
The full line from Eminem in 'A Kiss' - which is released under Eminem's Bad Meets Evil side project with Royce Da 5' 9"- is: "Tell Lady Gaga she can quit her job at the post office/ She's still a male lady."
"Wouldn't f**k her with her d**k/ You heard it/ The verdict's in."
'Judas' hitmaker Gaga has mocked rumours she was actually a hermaphrodite - started in 2009, after a photo of her in England seemingly showed a bulge in her underwear - by saying she was planning to wear a fake penis on stage.
She said: "I want to wear a d**k strapped to my vagina. We all know that one of the biggest talking points of the year was that I have a d**k, so why not give them what they want?"
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