The Eagles singer/songwriter has labelled Frank a ''talentless little p***k'' after previously threatening to sue him when the rapper used The Eagles' song 'Hotel California' on his track 'American Wedding' without permission.
He said: ''I didn't think he was cool. I thought he was a talentless little p***k. And I still do.
''Some of these young kids have grown up in a world that doesn't understand or respect copyright material or intellectual property. They look at songs as interactive playthings.''
When asked by The Guardian if he would be more comfortable if a more established artist, such as Kanye West, sampled his music, Don said: ''No, I'd be just as p****d off. I don't like him, either.''
And Don also hit out at Kanye's claims that he plans to run for President of the United States in 2020.
The musician - who has a new country album 'Cass County' coming out - scoffed: ''He won't be president. He's either incredibly arrogant or incredibly insecure, or some combination of the two.''
His new album Underneath It All is out now.