Worn by the legendary beauty when she collected her Best Actress Academy Award for her role in 'Roman Holiday' in 1952, the lace gown - adapted from a version designed by Edith Head - is expected to fetch up to £60,000 when it goes under the hammer later this month.
The ivory full-skirted dress - known as Audrey's "lucky" garment - was originally given to the late star's mother, Ella van Heemstra, who later gave it to a family friend living In America.
However, the gown had been stashed away at the bottom of a wardrobe until they recently decided to sell it.
The sale - which will take place on November 29 at La Galleria in London's Pall Mall - will be hosted by Kerry-Anne Auctions, the same company that sold the infamous sheer dress worn by Catherine, Duchess of Cambridge, when she participated in a university fashion show in 2002.
In March, the see-through dress - which is said to have first attracted Prince William to his future wife - sold for £78,000 at auction after attracting a huge amount of interest from all over the world.