The Against Me! singer, who is transgender, was upset by the promo for the Canadian group's gay-rights anthem when she realised a heterosexual actor was playing dress-up, rocking out onstage with the band at the Coachella festival in California.
Garfield shaved his head and wore a wig and a dress in the video.
In a Twitter.com rant, posted on Thursday (22May14), Grace wrote, "Dear @arcadefire, maybe when making a video for a song called 'We Exist' you should get an actual 'Trans' actor instead of Spider-Man?"
The Against Me! star, born Tom Gabel, came out about her gender dysphoria in 2012.
Arcade Fire have yet to respond to the tweet.